STUDI AWAL IDENTIFIKASI ASAM LAKTAT DARI AMPAS UMBI DAHLIA

Abstract

ABSTRACT

The waste of dahlia tuber can be used as raw material for lactic acid production through fermentation process. The aim of this research was to study lactic acid production from glucose which is converted from dahlia tuber waste by hydrolyis process. Acid hydrolysis process performed on the samples using 10% sulfuric acid (H₂SO₄). The hydrolyzate then fermented using Lactobacillus bulgaricus lasted for 120 hours at 30°C with a speed of 250 rpm. The results showed that glucose consumption and lactic acid product were increased following increasing fermentation age and happened at all of treatment medium. The highest lactic acid product was 0,4% on medium with pH 4 controlling at 120 hours fermentation. Lactic acid product on medium with pH 5, 6 dan 7 controlling at 120 hours fermentation were 0,36%; 0,25%; dan 0,18%.

Keywords:dahlia tuber waste, glucose, Lactobacillus bulgaricus, fermentation, lactic acid.